Detection and quantification of <i>Bacillus cereus</i> and its spores in raw milk by qPCR, and distinguish <i>Bacillus cereus</i> from other bacteria of the genus <i>Bacillus</i>

Abstract Introduction Raw milk is the basic raw material of dairy products. Bacillus cereus (B. cereus) a typical conditional pathogenic bacteria and cold-phagocytic spoilage in milk. Materials Methods In this study, quantitative polymerase chain reaction (qPCR) method for detecting B. was established. The specificity verified by using other bacteria; sensitivity evaluated preparing recombinant plasmids simulated contaminated samples; applicability pure spore DNA. actual sample detection completed established qPCR method. Results study can specifically detect limit as low 200 CFU/mL, quantification ranged from 2×102 to 2×108 amplification efficiency 96.6%. Conclusions distinguish bacteria, DNA be used object. This has advantages strong specificity, high sensitivity, wide application range, short time, which expected applied industry.